RNA-Seq analysis workflow using High Performance Computing

• Speakers: Dr Prasanth Sivakumar, Dr Mansoor Saqi, Dr Domenico Cozzetto, Ms Rossella Rispoli
• Location: online
• Book on Skillsforge, or for clinical staff without a skillsforge login, use our Research Methods form
• Part one on 18 March, and Part two on 25 March (both 09:00 – 12:00) 
• Attendance at An Introduction to the use of the Rosalind High Performance Computing System is required

Course description

RNA sequencing is a popular next generation sequencing method that enables the identification and quantification of both known and novel transcripts across the entire transcriptome, but requires several complex and computationally-intensive processing steps. This course gives an introduction into how researchers with a beginner’s understanding on the command line interface can gain experience with such methods on Rosalind, King’s high performance computing system. RNA-Seq data will be processed and analysed hands-on by working through a guided example using a sample of real published datasets.

Pre-requisites:

• Attendance at An Introduction to the use of the Rosalind High Performance Computing System.
• Basic knowledge of working on the command line is required.
• An account on Rosalind is necessary and access must be fully set up prior to attending this course.
• Basic understanding of RNA sequencing is not necessary but would be beneficial.

Who can attend?

The courses are free for health care professionals employed by organisations associated with the National Institute of Health Research Biomedical Research Centre at Guy’s and St Thomas’ and King’s College London:

• King’s College London
• Guy’s and St Thomas’ NHS Foundation Trust
• Royal Brompton and Harefield hospitals
• South London and the Maudsley NHS Foundation Trust
• King’s College Hospital NHS Foundation Trust

Places are booked on a first come, first served basis. If the module is full, your name will be placed on a waiting list and if a place becomes available you will contacted at the latest 72 hours beforehand.

Non Attendance Policy

The courses in this programme are very popular and are often oversubscribed. If you are unable to attend a course you are booked on to you must notify us 72 hours in advance so we can offer the place to someone else.

If you do not attend on the day and are unable to provide a satisfactory explanation you may be removed from future course bookings.

For further information please contact the NIHR Guy’s and St Thomas’ BRC Research Training Programme at BRCTraining@gstt.nhs.uk.