The BRC Flow Core is open to members of our NIHR Biomedical Research Centre, King’s College London investigators and external users on a fee for service basis. The core accepts contract work from external customers. For enquiries please contact Susanne.Heck@gstt.nhs.uk.

Trained internal users will be able to use the flow cytometry core laboratory 24 hours a day, 7 days a week. External users will not be given independent access and can use the facility only during regular working hours (Monday- Friday, 09.00 – 17.00).

All instruments can be reserved on a first-come, first-served basis via online calendars. You can access these calendars on the relevant instrument webpages once you have received relevant training and registered with iLabs to use the facilities.

Registration with iLabs

First time users must register with iLabs (PDF 235Kb), our online booking and invoicing system and complete a BRC Flow Core registration form (Word 36Kb) form which needs to be sent to BRCFlowCore@gstt.nhs.uk

All users wishing to use the facility analysers independently must obtain relevant training prior to using these facilities for the first time. Training is booked via iLabs (see “request Services” tab).

Please also refer to Gaining Access for further instructions.

Users will obtain card access to the flow lab which must be authorised by either Susanne Heck susanne.heck@gstt.nhs.uk Tel 020 7188 1184 or Stephen Barnett Stephen.Barnett@gstt.nhs.uk, CRF business manager, Tel 020 7188 1103.

Access to user operated instruments

All users wishing to use the BD analysers on their own, must first attend our basic training course. Once this training has been completed, a user name and password will be issued which allows timeslots to be booked on the instrument calendars.

Access to the ARIA 1 sorter for independent after hour and weekend use will be given only to advanced users that have undergone BD ARIA operator training and in prior agreement with the Head of the BRC Flow Core.

To use the Luminex FlexMap3D users will have to complete online training and a brief introduction to using the instrument, provided by flow cytometry laboratory staff. To use the Amnis ImageStreamMkII we offer monthly training sessions. All training is booked via iLabs (see “Request Services” tab)

Access to operator-run instruments

Two of our ARIA sorters as well as our CyTOF and Helios mass cytometers are exclusively operated by flow cytometry core staff. To book sorting time please submit  a completed a sort request in iLabs (see “Request services” tab for appropriate form). The facility will notify you via ilabs when your sort is booked. Flow Cytometry laboratory staff will assign all sorting times including those for self-operators of ARIA1. Users will be able to see (but not book or alter) the sorter and mass cytometer schedules. Please DO NOT email the core/individual team members to request sort time, we need a record on ilabs for all activities and will not set up bookings that reach us via email only.