Overview

The BD FACSCanto™ II system is specifically designed to support a wide range of basic flow cytometric applications such as immunophenotyping, cell cycle analysis and apoptosis.

The BRC flow Core offers two BD FACSCanto™ II systems with 3 lasers (405 nm, 488 nm, 633 nm) and an automated sample loading device (40 tube carousel). Our CANTOs allow the use of up to eight fluorochromes. A barcode reader is available as an extra option. The BD FACSCANTO II™ analysers use FACSDIVA software for acquisition and analysis.

BD FACSCANTO II

Configuration of the BD FACSCanto™ II analysers

Both CANTO analysers are 3 laser, 10 parameter instruments (405 nm (violet), 488nm (blue), 633 nm (red)) and are equipped with a carousel loader. Laser, detector and filter configuration are identical on both systems:
4 Blue/2 Red/2 Violet:

Canto 1+2

Canto 12 Cropped

Note: The Flow core laboratory owns a large set of optional filters as well, but they are not routinely installed on the cytometer. If you require a different filter configuration for a specific fluorochrome, please inform the facility staff in advance.  Obtaining filters for novel dyes may take several weeks. Due to the fast growing number of dyes on the market the above list is by no means exhaustive, please ask the FCL staff if you are unsure which filter to use.

Booking

These analysers are available 24 hours, 7 days a week except during weekly maintenance and repair periods.

Before an investigator can book and use the equipment, they must attend training and register with iLabs (PDF 235Kb).