Overview

The BRC Flow Core offers a wide range of training classes, which are partially open to external customers as well. All users of the Flow Cytometry Core facility must attend relevant training prior to first use. Core analysers are in general run as self service instruments. There is only very limited availability for staff assistance to run samples. This service must be booked in advance and incurs an additional fee.

Before attending any of our training classes, users must

  1. register with iLabs (PDF 235Kb)
  2. send a completed Flow core registration form to BRCFlowCore@gstt.nhs.uk

Basic training classes

Overview: Basic training is mandatory for all new users wishing to access the BD analysers (CANTO/Fortessa/Symphony).
Content: The session consists of an introduction to flow cytometry followed by a practical hands on training session on the instruments.
Frequency: Classes will be held on a Friday once every month from 10.00 to 14.30 (30 minute break – please bring your own lunch)
Cost and eligibility: Basic training is charged at £100 per trainee and is open to internal core users only.
Location: 15th floor, Tower Wing, Guy’s Hospital
Class size: Classes are limited to eight participants.

Advanced training classes

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Overview: All users of advanced specialist analysers must obtain training on the respective platform prior to first use. All training sessions consist on an introduction into the technology followed by a hands-on demonstration at the instrument.

 

ImageStream Training

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It is essential that users have a good grasp of basic flow cytometry before attending this advanced training course, basics will not be covered in this class.

Frequency: Training will be held monthly on a Monday from 10.00 to 14.30 (30 minute break – please bring your own lunch)
Location:  15th floor of Guy’s Tower Wing
Class size: Classes are limited to six participants

High dimensional cytometry data analysis training classes

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Overview: Mass cytometry and high dimensional fluorescent flow cytometry approaches make it necessary to use advanced unsupervised data analysis tools such as viSNE, SPADE, CITRUS, Phenograph and others. The course is aimed at users with no or very limited prior knowledge on high dimensional data analysis and the CytoBank online platform. We will cover the standard data clean-up and analysis pipeline for mass cytometry and fluorescent flow cytometry data, basics of viSNE, SPADE and give an introduction to the CytoBank suite. Users will be provided with example data for analysis while guided by the course providers.

Frequency and duration: The course is offered runs for a full day (10.00 – 17.00), three times per year.
Cost and eligibility: The training is open to internal customers only on a fee for service basis.
Location: 15th floor of Guys Tower Wing.
Class size: We train in groups of six delegates per course.

Luminex Training

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It is essential that users have a good grasp of basic flow cytometry before attending this advanced training course, basics will not be covered in this class.

Frequency: Training will be arranged on an individual basis and lasts 2 hours
Location:  15th floor of Guy’s Tower Wing
Class size: Classes are limited to six participants
Cost: The training is open to internal and external customers on a fee for service basis

FlowJo software training classes

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Overview: The course is aimed at users with no or very limited prior FlowJo knowledge and those that know their way around the software but have advanced analysis requirements (large file numbers, complex multicolour panels, special applications) and will benefit from learning the tricks to a more effective use of the software. Users will be provided with example data for analysis while guided by the course providers.Training is done on FlowJo v10.

Frequency and duration: The course is offered three times a year on two consecutive days. One training session runs for a whole day (10.00 – 17.00) with lunch break,                   please bring your own lunch.
Cost and eligibility: The training is open to internal and external customers on a fee for service basis.
Location: 15th floor of Guys Tower Wing.
Class size: We train in groups of six delegates per course with two instructors present.