Overview

All users of the Flow Cytometry Core facility must attend relevant training prior to first use. Core analysers are in general run as self service instruments. There is only very limited availability for staff assistance to run samples. This service must be booked in advance and incurs an additional fee.

Before attending any of our training classes, users must

  1. register with iLabs (PDF 235Kb)
  2. send a completed BRC Flow Core registration form (Word 36Kb) form to BRCFlowCore@gstt.nhs.uk

Basic training classes

Overview: Basic training is mandatory for all new users wishing to access the BD analysers (CANTO/Fortessa).
Content: The session consists of an introduction to flow cytometry followed by a practical hands on training session on the instruments.
Frequency: Classes will be held on a Friday once every month from 10.00 to 14.00
Cost & eligibility: Basic training is charged at £100 per trainee and is open to core users only.
Location: 15th floor, Tower Wing, Guy’s Hospital
Class size: Classes are limited to eight participants.

The 2017 dates for the basic training course are as follows:

  • 20 January
  • 24 February
  • 24 March
  • 28 April
  • 19 May
  • 23 June
  • 21 July
  • 18 August
  • 22 September
  • 20 October
  • 17 November
  • 15 December

Important for new users

Please complete the following steps to sign up for the core before you come to training.

  1. Sign up for an ilabs account (steps 1 and 2)
  2. Request training via ilabs: Go to “request services”, choose “ 5.1 Basic Training” and submit to core. We will sign you onto the next available course/the date of your choice
  3. Submit a completed core lab registration form to :BRCFlowCore

Advanced training classes

Overview: All users of advanced specialist analysers must obtain training on the respective platform prior to first use.

  • Users must obtain instrument specific training before they will be allowed to independently use the Amnis ImageStreamMKII and the Luminex FlexMAP3D
  • CyTOF users will obtain training in the technology and panel design.

Cost: free of charge for internal users until further notice. All training is requested via ilabs (see “Request Services” tab).
Class size:
Luminex and CyTOF training sessions are arranged on an individual basis

Please complete the following steps to sign up for the core. Please note that you must complete this before you come to training.

  1. Sign up for an ilabs account (steps 1 and 2). If you have an ilabs account already proceed to step 2
  2. Request training via ilabs: Go to “request services”, choose appropriate training class and submit to core. We will sign you onto the next available course/the date of your choice.
  3. If new user:Submit a completed core lab registration form to :BRCFlowCoreTraining

ImageStream Training

It is essential that users have a good grasp of basic flow cytometry before attending this advanced training course, basics will not be covered in this class.

Frequency: Training will be held monthly on a Monday from 10.00 to 14.00
Location:  15th floor of Guy’s Tower Wing
Class size: Classes are limited to six participants
Cost: Image Stream training will be charged at £100 per trainee and is open to internal customers.

The remaining 2017 dates for the ImageStream training are as follows:

  • 23 January
  • 20 February
  • 27 March
  • 24 April
  • 30 May
  • 27 June
  • 24 July
  • 21 August
  • 25 September
  • 23 October
  • 20 November
  • 18 December

FlowJo software training classes

Overview: The course is aimed at users with no or very limited prior FlowJo knowledge and those that know their way around the software but have advanced analysis requirements (large file numbers, complex multicolour panels, special applications) and will benefit from learning the tricks to a more effective use of the software. Users will be provided with example data for analysis while guided by the course providers.

A structured FlowJo training module covering basic FlowJo skills and relevant frequently used special applications (e.g  proliferation, cell cycle). Training is done on FlowJo v10.
Frequency & duration: The course is offered over two days (10.00 – 17.00), three times per year.
Cost & eligibility: The training is open to internal and external customers on a fee for service basis.
Class size: We train in groups of six delegates per course with two instructors present.

At the end of this module users will be able to:

  • import raw flow cytometry data into FlowJo from any platform
  • annotate and organise files to aid basic analysis
  • use keywords for organising, analysing and batching medium to large data sets
  • carry out post-acquisition compensation
  • generate a statistical and graphical report of the experiment
  • analyse data from special applications such as proliferation and cell cycle.

The remaining 2017 dates for the FlowJo software training are as follows:

  • Monday 13 & Tuesday 14 March
  • Monday 17 & Tuesday 18 July
  • Monday 13 & Tuesday 14 November

If you have any questions regarding any of these training sessions please email BRCFlowCore@gstt.nhs.uk