The BD LSRFortessa™ cell analysers are high end analytical flow cytometers and offer unmatched performance, capabilities, and flexibility, utilising a revolutionary optical design. The electronics digitise signals 10 million times per second in 16,384 discrete levels. This expands channel resolution and increases detection sensitivity. Electronic dead time is eliminated; more precise fluorescent measurements can be made improving linearity, compensation, and better quantification of fluorescence measurements.

The BRC Flow Core offers two twenty parameter Fortessa instruments with five lasers (355 nm, 405 nm, 488 nm, 561 nm and 633 nm) and BD High Throughput Sampler (HTS) option. The BD LSRFortessa™ cell analysers use FACSDIVA software for acquisition and analysis.

A high end BD Fortessa X30 has been added to the core in 2015. This 5 laser 30 parameter instrument will allow deep immunophenotyping with fluorescent markers to a previously unprecedented level. For more information please contact us at BRCflowcore@gstt.nhs.uk.

Configuration of the Fortessa analysers

BRC SORP Fortessa 5 laser, 20 parameter SORP instruments (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), HTS option for 96 well plate acquisition. Laser, detector and filter configuration 3 Blue/3 Red/5 Violet /2 nUV/ 5 Yellow-Green:

Note: The Flow core laboratory owns a large set of optional filters as well, but they are not routinely installed on the cytometer. If you require a different filter configuration for a specific fluorochrome, please inform the facility staff in advance.  Obtaining filters for novel dyes may take several weeks. Due to the fast growing number of dyes on the market the above list is by no means exhaustive, please ask the Flow Core Laboratory staff if you are unsure which filter to use.

Booking

The analysers are available 24 hours a day, 7 days a week for use by investigators except during weekly maintenance and repair periods. For details of these, see the guidelines page.

Before an investigator can book and use the equipment, they must attend training and register with iLabs (PDF 235Kb).