The electronics digitise signals 10 million times per second in 16,384 discrete levels. This expands channel resolution and increases detection sensitivity. Electronic dead time is eliminated; more precise fluorescent measurements can be made improving linearity, compensation, and better quantification of fluorescence measurements.
Configuration of the Fortessa analysers
BRC SORP Fortessa 5 laser, 20 parameter SORP instruments (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), HTS option for 96 well plate acquisition. The BD LSRFortessa™ cell analysers use FACSDIVA software for acquisition and analysis.
Laser, detector and filter configuration 3 Blue/3 Red/5 Violet /2 nUV/ 5 Yellow-Green:
Note: The Flow core laboratory owns a large set of optional filters as well, but they are not routinely installed on the cytometer. If you require a different filter configuration for a specific fluorochrome, please inform the facility staff in advance. Obtaining filters for novel dyes may take several weeks. Due to the fast growing number of dyes on the market the above list is by no means exhaustive, please ask the FCL staff if you are unsure which filter to use.
The analysers are available 24 hours a day, 7 days a week for use by investigators except during weekly maintenance and repair periods. For details of these, see the guidelines page.