Overview

All sorters allow two and four way bulk sorting into a variety of tube sizes or can sort using Automated Cell Deposition Unit (ACDU) to multi-well plates or microscope slides. Index sorting and sample and collection tube cooling is available on all instruments.The BD FACSAria™ cell sorters use FACSDIVA software for acquisition and analysis.

image-flow-BD-ARIA-3-FUSION 2
image-flow-FACS-Aria

Sort slots can be requested by the user via ilabs on the respective sorter calendars. Only after facility review and acceptance of the requested time the booking will become binding. The flow cytometry laboratory staff will review all sort requests and strive to avoid large blocks of unused time to maximize access.  Before booking a sort for the first time you need to register with iLabs (PDF 235Kb) and send us a  form if you only use our sort service. To cancel a sort, please complete the sort cancellation form available in the “Request Services” tab in ilabs, do NOT email the team only as we will need a complete audit trail of all bookings.

Configurations of the BD FACSAria™ Cell Sorters

BRC Aria 1 sorter

3 laser, 12 parameter instrument (405 nm (violet), 488nm (blue), 633 nm (red). ACDU installed. Laser, detector and filter configuration 6 Blue/3 Red/3:

image-flow-ARIA-1-sorter2-table

Note: The Flow core laboratory owns a large set of optional filters as well, but they are not routinely installed on the cytometer. If you require a different filter configuration for a specific fluorochrome, please inform the facility staff in advance. Obtaining filters for novel dyes may take several weeks. Due to the fast growing number of dyes on the market the above list is by no means exhaustive, please ask the Flow Core Laboratory staff if you are unsure which filter to use.

Booking the BRC ARIA 1 sorter

The machine is facility operated during regular business hours and can be booked by all users during this time. Trained and approved users can also obtainafter hours and weekend access. (Monday to Friday earliest booking: 5:30 PM if no facility sorts are ongoing). BRC ARIA1 is therefore available 24/7.

Only advanced flow cytometry users can obtain after hour and weekend access to the self service BRC ARIA1sorter. To become a self-service user for BRC ARIA1, applicants must be approved by the Head of the BRC Flow Cytometry Platform and have a certificate of completing an official BD Aria operator training course. After approval, users will be issued with a username and password, which allows access to the sorter.

Flow core staff reserve the right to review and/or revoke access to the self-service ARIA if a user repeatedly fails to safely operate the sorter. FCL staff also reserve the right to review and/or alter the time booked on BRC ARIA1 to allow maximum access to the sorter for all users.

Self-service users are responsible for filling and emptying of all fluidics and for instrument setup and cleaning. All sorts are run routinely with sterile 1x PBS as sheath buffer.

BRC ARIA 2 and BRC ARIA 3 Fusion

5 laser, 20 parameter SORP instruments (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red). ACDU and Aerosol Management system installed. Laser/Detector and filter configuration (3 Blue/3 Red/5 Violet /2 nUV/ 5 Yellow-Green).

image-flow-ARIA2-and-3-table

Note: The Flow core laboratory owns a large set of optional filters as well, but they are not routinely installed on the cytometer. If you require a different filter configuration for a specific fluorochrome, please inform the facility staff in advance. Obtaining filters for novel dyes may take several weeks. Due to the fast growing number of dyes on the market the above list is by no means exhaustive, please ask the Flow Core Laboratory staff if you are unsure which filter to use.

Booking BRC ARIA 2 and BRC ARIA 3 Fusion

BRC ARIA2 and BRC ARIA3 Fusion are exclusively operated by flow cytometry laboratory staff. The instruments and operators are available for sorts Monday to Friday, 9am to 5pm. All sorts are run with sterile 1x PBS as sheath buffer.

All sort slots can be requested by the user via ilabs on the respective sorter calendars. Only after facility review and acceptance of the requested time the booking will become binding. The flow cytometry laboratory staff will review all sort requests and strive to avoid large blocks of unused time to maximize access.  Before booking a sort for the first time you need to register with iLabs (PDF 235Kb) and send us a Flow Platform registration form (24kb) if you only use our sort service. To cancel a sort, please complete the sort cancellation form available in the “Request Services” tab in ilabs, do NOT email the team only as we will need a complete audit trail of all bookings.