Overview

The ISX provides new insights into the co-localisation of proteins, cell-cell interactions, internalisation, targeted immunotherapy, parasitaology or cell cycle/cell death related questions.The technology is furthermore well suited for the study of extracellular vesicles such as exosomes.

Our ImageStream MarkII is equipped with 2 cameras providing 12 image channels and 4 collinear excitation lasers (405 nm, 488 nm, 560 nm, and 642 nm) as well as MultiMag (20x/40x/60x) and EDF (extended depth of field). Cellular imagery is split into its component colours through a unique spectral decomposition element. The result is a bright field image, a dark field image, and multiple fluorescence images of every cell. The ImageStream MKII uses the latest versions of INSPIRE software for acquisition and IDEAS software for analysis. The table below allows for an easy overview of possible fluorochrome combinations.

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Booking

To book the BRC ImageStream analyser please register with the iLabs (PDF 235Kb) booking system and submit a Flow Core Registration Form (23kb). First time users must attend our ImageStream training prior to first use. Please book a consultation session before starting your first experiment.