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Aria 1 and Aria 2

BD FACSAria II

The BD FACSAria™ II Cell Sorter is a benchtop high-speed flow cytometry cell sorter with a fixed-alignment cuvette flow cell and up to 5 air-cooled lasers. It allows for high-speed multicolour sorting with digital acquisition rates of up to 20,000 events per second.

The flow laboratory is equipped with two Aria sorters. Both can sort into two- and four-way bulk sorting devices for a variety of tube sizes or can sort using Automated Cell Deposition Unit (ACDU) to multi-well plates or microscope slides. The BD FACSAria™ II Cell Sorters use FACSDIVA software for acquisition and analysis.

Configurations of the BD FACSAria™ II Cell Sorters

ARIA 1

4 laser, 10 parameter instrument (355 nm (nUV), 405 nm (violet), 488nm (blue), 633 nm (red)), either the UV or the violet laser can be used, not both simultaneously. ACDU installed. Laser, detector and filter configuration 6 Blue/2 Red/2 Violet or nUV:

Laser

LP Mirror

BP Filter

Intended Dye

488 nm

 

 

 

 

 

optional

 

735 nm

780/60 nm

PE-Cy7

655 nm

695/40 nm

PE-Cy5.5, PerCP Cy5.5

610 nm

616/23 nm

PE-TexasRed

556 nm

585/42 nm

PE

502 nm

530/30 nm

FITC

Blank

488/10

SSC

655 nm

675/20 nm

Pe-Cy5, PerCP

 

633 nm

 

 

735 nm

780/60 nm

APC-Cy7

Blank

660/20 nm

APC

405 nm

505 nm

530/30

AmCyan

Blank

450/50 nm

Pacific Blue

351 nm

505 nm

530/30

Indo1

Blank

450/50 nm

DAPI/Hoechst

 

Note: The core laboratory owns the optional filters as well, but they are not routinely installed in the cytometer. If a user requires them for an experiment, please inform the facility staff in advance.

Using the Aria 1 sorter

The machine is facility operated during regular business hours and can be booked by trained and approved users for after hour and weekend access. BRC ARIA1 is therefore available 24/7.  

Only advanced flow cytometry users can obtain after hour and weekend access to the self service Aria1 Sorter.

To become a self-service user for ARIA1, applicants must be approved by the senior flow cytometry manager and have a certificate of completing an official BD Aria operator training course. After approval, users will be issued with a username and password, which allows access to the sorter. 

Flow cytometry laboratory staff reserve the right to review and/or revoke access to the self-service ARIA if a user repeatedly fails to safely operate the sorter. FCL staff also reserve the right to review and/or alter the time booked on ARIA1 to allow maximum access to the sorter for all users.

Users are responsible for filling and emptying of all fluidics and for instrument setup and cleaning. All sorts are run with sterile 1x PBS as sheath buffer. Users can provide their own sheath buffer if necessary but must inform the Flow Cytometry Laboratory at least 2 days in advance (also applies to self users).

The flow cytometry laboratory staff will assign all sorting times and strive to avoid large blocks of unused time to maximize access. To book sorting time on this instrument, please register with iLabs (PDF 60Kb) and complete the sort request form available in this booking system. 

 

ARIA 2 (Flow cytometry laboratory operated):

5 laser, 20 parameter SORP instrument (355 nm (nUV), 405 nm (violet), 488nm (blue), 561nm (Yellow-green), 633 nm (red)), allows simultaneous use of all lasers. ACDU and Aerosol Management system installed. Laser/Detector and filter configuration (3 Blue/3 Red/5 Violet /2 nUV/ 5 Yellow-Green):

Laser

LP Mirror

BP Filter

Intended Dye

488 nm

 

Optional

 

 

 

 

550 nm

575/26 nm

PE

505 nm

530/30 nm

FITC

Blank

4881/10 and pinhole

SSC

505 nm

525/50

FITC

495 nm

515/20 nm

EGFP

525 nm

545/35 nm

YFP

635 nm

670 LP

PerCP, PerCP-Cy5.5

561 nm

 

 

Optional

 

735 nm

780/60 nm

PE-Cy7

685 nm

710/50 nm

PE-Cy5.5

635 nm

660/20 nm

PE-Cy5

595 nm

610/20 nm

PE-TexasRed,mCherry

570 nm

585/15 nm

PE

750 nm

780/60 nm

PE-Cy7

635 nm

670/30 nm

PE-Cy5

 

633 nm

 

Optional

 

735 nm

780/60 nm

APC-Cy7

685 nm

710/50 nm

Alexa700

Blank

670/14

APC

750 nm

780/60 nm

APC-Cy7

690 nm

730/45 nm

Alexa700

Blank

670/30 nm

APC

405 nm

755 nm

780/60 nm

QDot 800

 

690 nm

710/50 nm

QDot 700

600 nm

610/20 nm

QDot 605

570 nm

585/42 nm

QDot 585

505 nm

525/50 nm

AmCyan

Blank

450/50 nm

Pacific Blue

351 nm

505 nm

530/30

Indo1

 

Blank

450/50 nm

DAPI/Hoechst

 

Note: The core laboratory owns the optional filters as well, but they are not routinely installed in the cytometer. If a user requires them for an experiment, please inform the facility staff in advance.

Booking Aria 2

The Aria 2 is exclusively operated by flow cytometry laboratory staff. The instrument and operators are available for sorts Monday to Friday, 9am to 5pm. 

All sorts are run with sterile 1x PBS as sheath buffer. Users can provide their own sheath buffer if necessary but must inform the Flow Cytometry Laboratory at least 2 days in advance.

The flow cytometry laboratory staff will assign sorting times and strive to avoid large blocks of unused time to maximize access. To book sorting time on this instrument, please register with iLabs (PDF 60Kb) and complete the sort request form available in this booking system.

 
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